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Registros recuperados: 6
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An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica ArchiMer
Lechable, Marion; Jan, Anne-cecile; Duchene, Axel; Uveira, Julie; Weissbourd, Brandon; Gissat, Loann; Collet, Sophie; Gilletta, Laurent; Chevalier, Sandra; Leclère, Lucas; Peron, Sophie; Barreau, Carine; Lasbleiz, Régis; Houliston, Evelyn; Momose, Tsuyoshi.
The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different...
Tipo: Text Palavras-chave: Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00656/76820/77996.pdf
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Biology and life table of Dirphia araucariae (Lepidoptera: Saturniidae): A herbivore of potentially high impact on Araucaria angustifolia Rev. Bras. Zool.
Zenker,Mauricio M.; Specht,Alexandre; Fronza,Edegar; Poletto,Graziela; Marcon,Fernanda; Formentini,Aline C.; Gedoz,Mateus.
The life-history and biology of Dirphia araucariae Jones, 1908, including its life fertility table, are here described. Moths were reared in the laboratory under controlled conditions on their host plant, Araucaria angustifolia (Bertoloni) O. Kuntze - Araucariaceae. We describe several life-history traits of the species, namely: developmental period, survival rate, growth rate, fertility, fecundity, sex ratio, cephalic capsule width, and pupal weight. Mean duration of life stages were: egg = 26.78 days; larva = 61.78 days; prepupa = 6.85 days; pupa = 62.46 days; adult = 8.37 days. We found statistically significant differences between sexes for adult, larval and pupal stage duration; larval stage was longer in females while pupal stage was longer in males....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Forest; Conservation biology; Developmental biology; Forest entomology; Pinheiro-do-Paraná; South America.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000200004
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Biology and morphology of Molippa cruenta (Lepidoptera: Saturniidae) in the laboratory Rev. Bras. Zool.
Specht,Alexandre; Formentini,Aline C.; Benedetti,Augusto J..
The goal of this contribution was to study the biology and morphology of all developmental stages of Molippa cruenta (Walker, 1855), a member of Hemileucinae (Saturniidae), endemic to southern Brazil and northeastern Argentina. Daily observations were carried out on individuals reared under controlled temperature (25 ± 1ºC), relative humidity (70 ± 10%) and photophase (14 hours of light). The duration and viability of each developmental phase, as well as morphological and behavioral aspects of each stage, were evaluated. Under laboratory conditions, the life-cycle of M. cruenta was completed in 150 days, with mean periods for egg, larva, pupa and adult phases being 35, 71.95, 37.85, 19.17 and 4.81 days, respectively. The larval phase included six instars,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Developmental biology; Hemileucinae; Larvae; Life-cycle.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000300005
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Morphologic analysis of developmental phases and gill ontogenesis in neotropical species Poecilia vivipara (Cyprinodontiformes: Poeciliidae) exposed to different salinities Rev. Bras. Zool.
Rocha,Thiago L.; Carvalho,Rodolfo; Yamada,Áureo T.; Sabóia-Morais,Simone M. T..
The present study evaluated and characterized the different phases of development and the cell types present in the gill epithelium of embryos, larvae, and fingerlings of guppy (Poecilia vivipara Bloch & Schneider, 1801) specimens exposed to water with salinity ranging from 0‰ to 20‰. The development of P. vivipara was classified into eight phases. The yolk was intensely eosinophilic, with glycidic and lipidic components between phases 1 to 7. After the development of their branchial epithelium, the presence of pavement cells, mitochondria-rich cells, and mucous cells (MCs) was observed. The understanding of ontogenesis indicates the sequence of cellular origin. Only type IV MCs located in the gill rays was detected from phase 3 through phase 7....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Developmental biology; Euryhalinity; Guppy; Mucous cells; Teleosts.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000400007
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Relationship of morphometrics, total carotenoids, and total lipids with activity and sexual and spatial features in Euphausia superba ArchiMer
Färber Lorda, Jaime; Ceccaldi, Hubert J..
Morphological differences associated with sex or stage, together with total lipids and carotenoids, were studied in Euphausia superba as possible indicators of physiological condition. E. superba displays sexual dimorphism during growth. A group of mature males, called Males II herein, has a greater abdominal length, suggesting that they are faster swimmers, a feature implying higher metabolic rates and a higher demand for protecting pigments like carotenoids. Mature Males II have proportionally lower lipids but higher total lipid-soluble carotenoids, a counterintuitive finding. Males II also have bigger eyes. Significant regressions with carotenoids were found for wet weight, abdominal length, and eye diameter. On a spatial analysis, population...
Tipo: Text Palavras-chave: Developmental biology; Ecology.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75471/76296.pdf
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X-Linked Retinitis Pigmentosa 2 Is a Novel Maternal-Effect Gene Required for Left-Right Asymmetry in Zebrafish ArchiMer
Desvignes, Thomas; Nguyen, Thaovi; Chesnel, Franck; Bouleau, Aurelien; Fauvel, Christian; Bobe, Julien.
Retinitis pigmentosa 2 (RP2) gene is responsible for up to 20% of X-linked retinitis pigmentosa, a severe heterogeneous genetic disorder resulting in progressive retinal degeneration in humans. In vertebrates, several bodies of evidence have clearly established the role of Rp2 protein in cilia genesis and/or function. Unexpectedly, some observations in zebrafish have suggested the oocyte-predominant expression of the rp2 gene, a typical feature of maternal-effect genes. In the present study, we investigate the maternal inheritance of rp2 gene products in zebrafish eggs in order to address whether rp2 could be a novel maternal-effect gene required for normal development. Although both rp2 mRNA and corresponding protein are expressed during oogenesis, rp2...
Tipo: Text Palavras-chave: Developmental biology; Egg developmental competence; Egg quality; Fish; Fish reproduction; Maternal-effect gene; Ndpk; Nme10; Oocyte; Oocyte-specific; Ovum; Teleost; Zygote.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00280/39099/37660.pdf
Registros recuperados: 6
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